ja 25 50 rotor (Danaher Inc)

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Danaher Inc ja 25 50 rotor
Ja 25 50 Rotor, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ja+25+50+rotor/JA-25%2E50+Fixed-Angle+Rotor/bio_rxiv__64898__2026__03__05__709893-158-22-24
Average 97 stars, based on 904 article reviews

ja 25 50 rotor - by Bioz Stars, 2026-10

97/100 stars

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Centrifugation:

Article Title: Genomic, Functional, and Evolutionary Insights into a Novel T7-like Phage B1 Infecting Multidrug-Resistant Enterobacter cloacae
Article Snippet: .. High-titer phage lysate (10 12 PFU) was first precipitated using 10% ( w / v ) PEG 8000 and 0.5 M NaCl at 4 °C for 12 h, followed by centrifugation at 15,000× g for 2 h at 4 °C using an Avanti JXN-26 centrifuge equipped with a JA-25.50 rotor (Beckman Coulter, Brea, CA, USA) to pellet the phage particles. ..

Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
Article Snippet: .. The lysate was clarified by centrifugation at 13,000 x g for 30 min at 4 °C using a Beckman centrifuge equipped with a JA-25.50 rotor (Beckman Coulter). .. The supernatant was centrifuged for 4 h at 35,000 rpm to collect the membrane fraction using a Beckman centrifuge equipped with a Type 45 Ti fixed-angle rotor (Beckman Coulter).

Article Title: Mechanism of SARS-CoV-2 resistance to nucleotide analog-based antivirals
Article Snippet: All proteins were overexpressed in E. coli BL21 Star (DE3) (ThermoFisher Scientific) at 17 °C for 18 h. Cells were resuspended in buffer A (50 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES), pH 7.5, 200 mM NaCl, 5% glycerol, 1 mM β-mercaptoethanol (β-ME), 20 mM imidazole) and lysed using a sonicator (QSonica). .. The cell lysate was cleared by centrifugation at 19,500 rpm using a JA-25.50 rotor (Beckman Coulter) for 1 h at 4 °C. .. The clarified cell lysate was loaded onto a HisTrap HP affinity chromatography column (Cytiva Life Sciences) and eluted through a linear gradient from 100% buffer A to 40% buffer A mixed with 60% buffer B (50 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol, 1 mM β-ME, 500 mM imidazole).

Article Title: Mechanism of SARS-CoV-2 resistance to nucleotide analog-based antivirals.
Article Snippet: All proteins were overexpressed in E. coli BL21 Star (DE3) (ThermoFisher Scientific) at 17 °C for 18 h. Cells were resuspended in buffer A (50 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES), pH 7.5, 200 mM NaCl, 5% glycerol, 1 mM β-mercaptoethanol (β-ME), 20 mM imidazole) and lysed using a sonicator (QSonica). .. The cell lysate was cleared by centrifugation at 19,500 rpm using a JA-25.50 rotor (Beckman Coulter) for 1 h at 4 °C. .. The clarified cell lysate was loaded onto a HisTrap HP affinity chromatography column (Cytiva Life Sciences) and eluted through a linear gradient from 100% buffer A to 40% buffer A mixed with 60% buffer B (50 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol, 1 mM β-ME, 500 mM imidazole).

other:

Article Title: Logic-Gated HSV-TK/GCV Suicide Gene Circuit for Triple-Negative Breast Cancer.
Article Snippet: HEK-293T cells were cotransfected with lentiviral package plasmid (psPAX2, Catalog no. 12260, Addgene), a plasmid encoding for VSV-G pseudotyping coat protein (pMD2G, Catalog no. 12259, Addgene), and target plasmid (37.5 μg total DNA) at a mass ratio of 2:1:2 using an optimized polyethyleneimine (PEI)-based protocol with 1.5 mL of a 3:1 PEI:DNA mixture (w/w).

Lysis:

Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
Article Snippet: .. Cell pellets were thawed, resuspended, and homogenized in lysis buffer (40 mM Tris pH 8.0, 400 mM NaCl, 10% glycerol), and centrifuged at 13,000 x g for 30 min at 4 °C in a Beckman centrifuge equipped with a JA-25.50 rotor (Beckman Coulter). .. The membrane fraction was isolated by centrifuging for 2.5 h at 186,000 x g using a Beckman ultracentrifuge equipped with a Type 45 Ti fixed-angle rotor (Beckman Coulter) and solubilized in 20 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol (v/v), 10 mM imidazole, and 1% (w/v) LMNG (Anatrace) for 2 h. The solubilized protein was incubated with nickel affinity resin (Thermo Fisher Scientific) at 4 °C for 1 h to maximize the binding to the target protein.

Homogenization:

Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
Article Snippet: Cell pellets were thawed, resuspended in 20 mM Na 2 HPO 4 (pH 7.3) and 300 mM NaCl (purification buffer) and supplemented with 1 mg/mL hen egg lysozyme (Sigma), EDTA, glycerol, protease inhibitors. .. Following homogenization, the lysate was centrifuged at 20,000 rpm for 20 min at 4 °C in a Beckman centrifuge equipped with a JA-25.50 rotor (Beckman Coulter). .. The clarified supernatant was loaded onto a column with pre-equilibrated amylose resins (E8021L, NEB).

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